Volume 1 Issue 2
Automated Image Analysis of Podocyte Desmin Immunostaining in a Rat Model of Sub-Acute Doxorubicin-Induced Glomerulopathy
Jing Ying Ma, Rachel Church, Justin Kanerva, Anton Bittner, James Eric McDuffie*
Remarkable histopathology findings first identified by light microscopic examination of hematoxylin and eosin stained tissues often warrant further characterization. Recent advancements in the field of molecular diagnostic pathology include fully automated whole-slide imaging. Such tools support best practices for digital imaging and quantification of positive immunohistochemical staining. Consequently, we developed a novel computational method to automatically detect total glomeruli in whole-tissue sections and to quantify areas and intensities of desmin immunolabeling in podocytes.
RDW is a Novel Prognostic Parameter in Diffuse Large B Cell Lymphoma in the Rituximab Era
Fatih Teker*, Yasemin Kemal, Bahiddin Yilmaz, Ahmet Siyar Ekinci, Yusuf Guney, Yüksel Bek, Mehmet Turgut, Idris Yucel
After the invention of rituximab, RCHOP became the standard regimen for Diffuse Large B cell lymphomas (DLBCL). Some patients still have a poor prognosis. Recent researches on Non-Hodgkin lymphoma has been related with prognostic biomarkers. Elevated RDW is accepted as a worse prognostic factor in some situations and diseases but the prognostic value has not been studied in DLBCL. The aim of this study was to investigate the prognostic role of RDW in DLBCL.
Review of the Literature: Sepsis and Neutrophil Cd64
Silvana Teixeira Dal Ponte*, Ana Alegretti, Gabriela Petitot Rezende, Giordanna Andrioli, Luciano Diogo, Luciano Zubaran Goldani, Pauline Simas Machado, Renato Seligman
Sepsis is a systemic inflammatory response against suspected or documented infection. In an infectious process, inflammation is triggered activating leukocytes, mainly neutrophils. CD64 is a surface antigen weakly expressed on non-activated mature neutrophils. When neutrophil CD64 is strongly expressed on neutrophils, it means neutrophils were activated, thus suggesting the presence of bacterial infection or acute fungal infection. A large number of biological substances has been investigated as candidate biomarkers and/or mediators of sepsis. Recent studies have focused on the investigation of neutrophil CD64 as a possible biomarker for the diagnosis of sepsis.
In Older Adults, Intervention Impacts the Association Between the Cortisol Awakening Response and Three Measures of Wellbeing: Meaningful Activities, Life Satisfaction and Perceived Control
Rand Wilcox*, Douglas A Granger, Sarah Szanton, Florence Clark
Studies report associations between the cortisol awakening response (CAR) and measures of psychological stress and wellbeing suggesting the possibility that individual differences in the CAR might influence which individuals respond to intervention programs aimed at promoting healthier lifestyle. This issue is studied in a randomized control trial in a large (N =460) ethnically diverse community sample of elder adults (M age 74.9; 66% female) using a cross-over design. Three key measures of wellbeing (i.e., meaningful activity, perceived control, and life satisfaction) were taken before and six months later. Some participants (N = 232) did not receive intervention the first six months, they acted as a control group, but after six months they received intervention and were measured again six months later.
Letter to the Editor
Biomarkers for Chagas Disease
Vitorino M Santos*, Lister A M Santos
Very important goal for researches about Chagas disease are reliable tools indicative of infection, before detectable pathology process. We read the manuscript of Pinho and Antas entitled Biomarkers for neglected Chagas disease where the eminent authors briefly, but clearly, highlighted the role of these necessary tools for diagnostic and therapeutic purposes. They also properly commented about major hurdles and challenges involving the routine utilization of biomarkers to assess the risk of disease among the more commonly affected individuals, who live in lowincome areas of Latin American countries.
Wnt and Notch Signaling Pathways are Involved in the Development of Aggressive Salivary Gland Neoplasms
UK Sinha MD, O. Gantz BS, J. Brooks MD, R Masood Ph D*
Tumors of the salivary glands are relatively uncommon malignancies, representing only 11% of oropharyngeal neoplasms in the United States. Salivary gland tumors consist of over 20 different histological subtypes and can affect both the major salivary glands (parotid, submandibular, sublingual), as well as minor salivary glands throughout the oral cavity and oropharynx. Salivary tumors present with a wide range of biological behavior, from benign (oncocytoma, cystadenoma, pleomorphic adenoma) to highly aggressive (low grade acinic cell carcinoma, adenoid cystic carcinoma, and high grade mucoepidermoid carcinoma) with wide spread invasion, local recurrence, and distant metastases.